PolysacDB O-polysaccharide 2041

From DrugPedia: A Wikipedia for Drug discovery

Contents 1 Carbohydrate Name 2 Carbohydrate Class 3 Source Microbe 4 Basic Structure 5 Proposed functions 6 Antigenic Nature used to produce antibodies 7 Carrier Name 8 Conjugation Method 9 Antibodies 10 Antibody type and class 11 Assay System 12 Cross-reactivity 13 Proposed epitopes 14 Proposed Utility 15 Weblink 16 External Links

[edit] Carbohydrate Name

O-polysaccharide

[edit] Carbohydrate Class

O-polysaccharide [LPS]

[edit] Source Microbe

Pseudomonas aeruginosa O5

[edit] Basic Structure

The LPS consists of three regions, namely O-polysaccharide, core oligosaccharide and lipid A. The core region of LPS is divided into the outer core and inner core. The inner core region consists of 2-keto-3-deoxyoctonic acid (KDO), heptose, phosphate, and probably ethanolamine. The outer core region commonly contains D-glucose, L-rhamnose, D-galactosamine, and L-alanine. The O-antigens consist of a trisaccharide repeating unit which consists of two uronic acid derivatives and one N-acetyl fucosamine residue

[edit] Proposed functions

N/A

[edit] Antigenic Nature used to produce antibodies

Formalin killed whole cells

[edit] Carrier Name

nil

[edit] Conjugation Method

nil

[edit] Antibodies

Mab MF15-4

[edit] Antibody type and class

IgM

[edit] Assay System

ELISA, immunoblotting, ouchterlony immunodiffusion

[edit] Cross-reactivity

This Mab was specific to serotype O5

[edit] Proposed epitopes

The epitope consisted of N-acetyl fucosamine residues with an αglycosidic linkage and a minimum of six O repeats of serotype O5 polysaccharide

[edit] Proposed Utility

This Mab helps us shed some light on the nature of O-specific and common epitopes in the LPS of P. aeruginosa

[edit] Weblink

PubMed Central

[edit] External Links

• BCSDB Structural Detail

• Link to PolysacDB database