PolysacDB Inner core Lipopolysaccharide 1091

From DrugPedia: A Wikipedia for Drug discovery

Contents 1 Carbohydrate Name 2 Carbohydrate Class 3 Source Microbe 4 Basic Structure 5 Proposed functions 6 Antigenic Nature used to produce antibodies 7 Carrier Name 8 Conjugation Method 9 Antibodies 10 Antibody type and class 11 Assay System 12 Cross-reactivity 13 Proposed epitopes 14 Proposed Utility 15 Weblink 16 External Links

[edit] Carbohydrate Name

Inner core Lipopolysaccharide

[edit] Carbohydrate Class

Core oligosaccharide [LPS]

[edit] Source Microbe

Escherichia coli F653

[edit] Basic Structure

It consists of the uncommon sugars L-glycero-D mannoheptose (Hep) and 3-deoxy-D-manno-octulosonic acid (Kdo) along with Lipid A

[edit] Proposed functions

N/A

[edit] Antigenic Nature used to produce antibodies

Heat killed bacteria

[edit] Carrier Name

nil

[edit] Conjugation Method

nil

[edit] Antibodies

Mab WN1 222-5

[edit] Antibody type and class

IgG2a

[edit] Assay System

ELISA, immunoblotting, passive hemolysis assay

[edit] Cross-reactivity

WN1 222-5 binds to all E. coli clinical isolates tested so far (79 blood isolates, 80 urinary isolates, and 21 fecal isolates), Salmonella LPS, Shigella LPS and to some Citrobacter, Enterobacter, and KiebsieUla isolates

[edit] Proposed epitopes

The epitope consists of the inner core

[edit] Proposed Utility

In vitro, this Mab inhibits the Limulus amoebocyte lysate assay activity of S-form and R-form LPS in a dose-dependent manner and it neutralizes the LPS-induced release of clinically relevant monokines (interleukin 6 and tumor necrosis factor). In vivo, WN1 222-5 blocks endotoxin-induced pyrogenicity in rabbits and lethality in galactosamine-sensitized mice

[edit] Weblink

[http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC281087 &tool=pmcentrez&rendertype=abstract PubMed Central]

[edit] External Links

• IEDB Epitopic Detail

• Link to PolysacDB database