PolysacDB Inner core Lipopolysaccharide 1091
From DrugPedia: A Wikipedia for Drug discovery
[edit] Carbohydrate Name
Inner core Lipopolysaccharide
[edit] Carbohydrate Class
Core oligosaccharide [LPS]
[edit] Source Microbe
Escherichia coli F653
[edit] Basic Structure
It consists of the uncommon sugars L-glycero-D mannoheptose (Hep) and 3-deoxy-D-manno-octulosonic acid (Kdo) along with Lipid A
[edit] Proposed functions
N/A
[edit] Antigenic Nature used to produce antibodies
Heat killed bacteria
[edit] Carrier Name
nil
[edit] Conjugation Method
nil
[edit] Antibodies
Mab WN1 222-5
[edit] Antibody type and class
IgG2a
[edit] Assay System
ELISA, immunoblotting, passive hemolysis assay
[edit] Cross-reactivity
WN1 222-5 binds to all E. coli clinical isolates tested so far (79 blood isolates, 80 urinary isolates, and 21 fecal isolates), Salmonella LPS, Shigella LPS and to some Citrobacter, Enterobacter, and KiebsieUla isolates
[edit] Proposed epitopes
The epitope consists of the inner core
[edit] Proposed Utility
In vitro, this Mab inhibits the Limulus amoebocyte lysate assay activity of S-form and R-form LPS in a dose-dependent manner and it neutralizes the LPS-induced release of clinically relevant monokines (interleukin 6 and tumor necrosis factor). In vivo, WN1 222-5 blocks endotoxin-induced pyrogenicity in rabbits and lethality in galactosamine-sensitized mice
[edit] Weblink
[http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC281087 &tool=pmcentrez&rendertype=abstract PubMed Central]
