PolysacDB Lipopolysaccharide 2501
From DrugPedia: A Wikipedia for Drug discovery
Carbohydrate Name
Lipopolysaccharide
Carbohydrate Class
Lipopolysaccharide
Source Microbe
Vibrio cholerae 01 Inaba 569B strain
Basic Structure
The O-antigen part consists of (1-->2)-α-linked 4-amino-4,6-dideoxy-D-mannose (perosamine) whose amino group is acylated with 3-deoxy-L-glycero-tetronic acid. The terminal sugar in the Inaba O-antigen has a hydroxyl at the 2' position
Proposed functions
V. cholerae lipopolysaccharide (LPS), a critical component of the outer membrane that is required for virulence, is a known target for immune responses following infection or immunization. Antibodies specific for V. cholerae LPS are correlated with protection against cholera
Antigenic Nature used to produce antibodies
Killed cells
Carrier Name
nil
Conjugation Method
nil
Antibodies
Mab F7P52C
Antibody type and class
IgG2a
Assay System
enzyme-linked immunosorbent assay (ELISA), immunoblotting and fluorescein isothiocyanate (FITC) immunofluorescence tests
Cross-reactivity
This Mab cross-reacted with LPS extracted from the same species, including V. alginolyticus, V. anguillarum, V. cholerae 01, V. firnissii, V. ordalii, and V. parahaemolyticus, but did not cross-react with other species within the family Vibrionaceae (i.e., Aeromonas, Photobacterinum, and Plesiomonas species) or other gram-negative bacteria
Proposed epitopes
N/A
Proposed Utility
The use of the Mabs can be in blind tests and diagnosis of clinical isolates
