PolysacDB Lipopolysaccharide 2501
From DrugPedia: A Wikipedia for Drug discovery
[edit] Carbohydrate Name
Lipopolysaccharide
[edit] Carbohydrate Class
Lipopolysaccharide
[edit] Source Microbe
Vibrio cholerae 01 Inaba 569B strain
[edit] Basic Structure
The O-antigen part consists of (1-->2)-α-linked 4-amino-4,6-dideoxy-D-mannose (perosamine) whose amino group is acylated with 3-deoxy-L-glycero-tetronic acid. The terminal sugar in the Inaba O-antigen has a hydroxyl at the 2' position
[edit] Proposed functions
V. cholerae lipopolysaccharide (LPS), a critical component of the outer membrane that is required for virulence, is a known target for immune responses following infection or immunization. Antibodies specific for V. cholerae LPS are correlated with protection against cholera
[edit] Antigenic Nature used to produce antibodies
Killed cells
[edit] Carrier Name
nil
[edit] Conjugation Method
nil
[edit] Antibodies
Mab F7P52C
[edit] Antibody type and class
IgG2a
[edit] Assay System
enzyme-linked immunosorbent assay (ELISA), immunoblotting and fluorescein isothiocyanate (FITC) immunofluorescence tests
[edit] Cross-reactivity
This Mab cross-reacted with LPS extracted from the same species, including V. alginolyticus, V. anguillarum, V. cholerae 01, V. firnissii, V. ordalii, and V. parahaemolyticus, but did not cross-react with other species within the family Vibrionaceae (i.e., Aeromonas, Photobacterinum, and Plesiomonas species) or other gram-negative bacteria
[edit] Proposed epitopes
N/A
[edit] Proposed Utility
The use of the Mabs can be in blind tests and diagnosis of clinical isolates
