PolysacDB Lipopolysaccharide 2314
From DrugPedia: A Wikipedia for Drug discovery
Revision as of 06:13, 8 April 2010
Carbohydrate Name
Lipopolysaccharide
Carbohydrate Class
Lipopolysaccharide
Source Microbe
Shigella flexneri serotype 2b
Basic Structure
Chemically, the O-antigen is a polysaccharide chain of variable length, built up by repetitive sequences of tetra- to hexasaccharides. It consists of a basic tetrasaccharide repeating unit consisting of three rhamnoses and one N-acetylglucosamine was common to all S. flexneri strains (except serotype 17): -2)-α-L-Rhap-(1-->2)-α-L-Rhap-(1-->3)-α-L-Rhap-(1-->3)-β-D-GlcpNAc-(1-->
Proposed functions
LPS plays an important virulence role based on its antiphagocytic and endotoxic properties
Antigenic Nature used to produce antibodies
Heat killed cells
Carrier Name
nil
Conjugation Method
nil
Antibodies
Mab MASF(7,8)-l
Antibody type and class
IgG3
Assay System
ELISA, agglutination tests
Cross-reactivity
This Mab showed excellent specificity for the 7,8 antigenic determinant [S. flexneri serotypes 2b, 3a, 5b, and X]
Proposed epitopes
This Mab apparently interact with the α-D-glucose p(1-->4)-α-L-rhamnosyl pIII epitope
Proposed Utility
This Mab can be used for detection and identification of shigella flexneri species
