PolysacDB Lipopolysaccharide 1069

From DrugPedia: A Wikipedia for Drug discovery

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Revision as of 06:03, 8 April 2010

Contents 1 Carbohydrate Name 2 Carbohydrate Class 3 Source Microbe 4 Basic Structure 5 Proposed functions 6 Antigenic Nature used to produce antibodies 7 Carrier Name 8 Conjugation Method 9 Antibodies 10 Antibody type and class 11 Assay System 12 Cross-reactivity 13 Proposed epitopes 14 Proposed Utility 15 Weblink 16 External Links

Carbohydrate Name

Lipopolysaccharide

Carbohydrate Class

Lipopolysaccharide

Source Microbe

Escherichia coli C600

Basic Structure

N/A

Proposed functions

N/A

Antigenic Nature used to produce antibodies

whole formalinized E. coli C600 cells

Carrier Name

nil

Conjugation Method

nil

Antibodies

Mab 786

Antibody type and class

IgG3

Assay System

ELISA and immunoblotting

Cross-reactivity

This antibody cross-reacted with LPS core types K-12, R4, and R2 but did not cross-react with Rl LPS and R3 LPS,

Proposed epitopes

The epitope was proposed to consist of trisaccharide sequence Hexp(1->2)-α-D -Glcp(1->3)α-D-Glcp->(Hepp) at the inner region of the outer core, in which the heptose-distal glucose is immunodominant

Proposed Utility

This Mab may be used for the detection of LPS core structures but also for analysis of the molecular forms resolved on polyacrylamide gels (banding patterns)

Weblink

[http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC261975 &tool=pmcentrez&rendertype=abstract PubMed Central]

External Links

• Link to PolysacDB database