PolysacDB Glucurunoxylomannan 2640

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Current revision (05:54, 9 September 2010) (edit) (undo) Raghavagps (Talk | contribs) m (1 revision)

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Contents 1 Carbohydrate Name 2 Carbohydrate Class 3 Source Microbe 4 Basic Structure 5 Proposed functions 6 Antigenic Nature used to produce antibodies 7 Carrier Name 8 Conjugation Method 9 Antibodies 10 Antibody type and class 11 Assay System 12 Cross-reactivity 13 Proposed epitopes 14 Proposed Utility 15 Weblink 16 External Links

[edit] Carbohydrate Name

Glucurunoxylomannan

[edit] Carbohydrate Class

Miscellaneous

[edit] Source Microbe

Cryptococcus neoformans strain 24067

[edit] Basic Structure

A linear α(1-->3)- linked mannan backbone singly substituted with non-reducing β(1-->2) xylose and β(1-->2) glucuronic acid side branches ; O-acetyl groups are present at C-6 of the mannosyl residues

[edit] Proposed functions

Antiphagocytic ; an important virulence factor

[edit] Antigenic Nature used to produce antibodies

Glycoconjugates

[edit] Carrier Name

Diphtheria toxoid

[edit] Conjugation Method

5 mg of GXM (strain 24067) was activated with 5 mg of CDAP (1-cyano-4-dimethylaminopyridinium tetrafluoroborate) and conjugated to Diphtheria toxoid

[edit] Antibodies

Mab G15

[edit] Antibody type and class

IgM

[edit] Assay System

ELISA and immunofluorescence

[edit] Cross-reactivity

This antibody cross-reacted with Serotypes A and D in whole cell ELISA and immunofluorescence. Did not cross-react with Serotype A glucurunoxylomannan alone

[edit] Proposed epitopes

N/A

[edit] Proposed Utility

This MAb mediated complement deposition on solid phase GXM by ELISA. Administration of Mab G15 also prolonged the survival of C. neoformans

[edit] Weblink

PubMed

[edit] External Links

• Link to PolysacDB database