PolysacDB Glucurunoxylomannan 2640
From DrugPedia: A Wikipedia for Drug discovery
[edit] Carbohydrate Name
Glucurunoxylomannan
[edit] Carbohydrate Class
Miscellaneous
[edit] Source Microbe
Cryptococcus neoformans strain 24067
[edit] Basic Structure
A linear α(1-->3)- linked mannan backbone singly substituted with non-reducing β(1-->2) xylose and β(1-->2) glucuronic acid side branches ; O-acetyl groups are present at C-6 of the mannosyl residues
[edit] Proposed functions
Antiphagocytic ; an important virulence factor
[edit] Antigenic Nature used to produce antibodies
Glycoconjugates
[edit] Carrier Name
Diphtheria toxoid
[edit] Conjugation Method
5 mg of GXM (strain 24067) was activated with 5 mg of CDAP (1-cyano-4-dimethylaminopyridinium tetrafluoroborate) and conjugated to Diphtheria toxoid
[edit] Antibodies
Mab G15
[edit] Antibody type and class
IgM
[edit] Assay System
ELISA and immunofluorescence
[edit] Cross-reactivity
This antibody cross-reacted with Serotypes A and D in whole cell ELISA and immunofluorescence. Did not cross-react with Serotype A glucurunoxylomannan alone
[edit] Proposed epitopes
N/A
[edit] Proposed Utility
This MAb mediated complement deposition on solid phase GXM by ELISA. Administration of Mab G15 also prolonged the survival of C. neoformans
