From DrugPedia: A Wikipedia for Drug discovery

Rv3710
Name
2-isopropylmalate synthase (leuA )
Identifiers
Swiss Prot	P96420
Genbank	885092
PDB	1SR9 3FIG, 1SR9
Chemical data
Formula	C15H10ClN3O3S [1]
Mol. wt.	70083 Da
Pharmacokinetic data
Bioavailability	?
Solubility	?
Isoelectric-Point	4.79

Contents 1 General 1.1 Protein Sequence 2 Human Homologue Blast Result 3 Alergen Protein 4 Bacterial Toxin Prediction 5 Subcellular Location 6 Antigens 6.1 B cell Epitopes 6.1.1 BCEpred Analysis 6.1.2 ABCpred Analysis 6.1.3 IEDB Analysis 7 MHC Class-I Binder 7.1 nHLAPred Analysis 7.2 IEDB Analysis 8 MHC Class-II Binder 8.1 Propred Analysis 8.2 NetMHC-II Analysis 9 External Links

[edit] General

INVOLVED IN LEUCINE BIOSYNTHESIS (AT THE FIRST STEP). CATALYZES CONDENSATION OF ACETYL-CoA AND 2-OXOISOVALERATE TO FORM 2-ISOPROPYLMALATE SYNTHASE [CATALYTIC ACTIVITY: 3-CARBOXY-3-HYDROXY-4-METHYLPENTANOATE + CoA = ACETYL-COA + 3-METHYL-2-OXOBUTANOATE + H(2)O].

Essential gene by Himar1-based transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003) mutants available at TARGET website

[edit] Protein Sequence

>Rv3710, TB.seq 4153737:4155668 MW:70083 VTTSESPDAYTESFGAHTIVKPAGPPRVGQPSWNPQRASSMPVNRYRPFAEEVEPIRLRNRTWPDRVIDRAPLWCAVDLR DGNQALIDPMSPARKRRMFDLLVRMGYKEIEVGFPSASQTDFDFVREIIEQGAIPDDVTIQVLTQCRPELIERTFQACSG APRAIVHFYNSTSILQRRVVFRANRAEVQAIATDGARKCVEQAAKYPGTQWRFEYSPESYTGTELEYAKQVCDAVGEVIA PTPERPIIFNLPATVEMTTPNVYADSIEWMSRNLANRESVILSLHPHNDRGTAVAAAELGFAAGADRIEGCLFGNGERTG NVCLVTLGLNLFSRGVDPQIDFSNIDEIRRTVEYCNQLPVHERHPYGGDLVYTAFSGSHQDAINKGLDAMKLDADAADCD VDDMLWQVPYLPIDPRDVGRTYEAVIRVNSQSGKGGVAYIMKTDHGLSLPRRLQIEFSQVIQKIAEGTAGEGGEVSPKEM WDAFAEEYLAPVRPLERIRQHVDAADDDGGTTSITATVKINGVETEISGSGNGPLAAFVHALADVGFDVAVLDYYEHAMS AGDDAQAAAYVEASVTIASPAQPGEAGRHASDPVTIASPAQPGEAGRHASDPVTSKTVWGVGIAPSITTASLRAVVSAVN RAAR

[edit] Human Homologue Blast Result

subject ids	% identity	% positives	alignment length	evalue
sp|P14679.3	23	38	100	0.37
sp|P60852.1	25	39	121	2.5
sp|Q8N2Y8.2	27	41	97	5.4
sp|Q9NQ90.2	31	45	73	6.6
sp|Q8N159.1	28	44	76	7.5

[edit] Alergen Protein

Link to Algpred
Allergen (By IgE method)

[edit] Bacterial Toxin Prediction

Link to btxpred
No Hit Fountd by btxpred server.

[edit] Subcellular Location

Link to TBpred
Cytoplasmic

[edit] Antigens

No Hit Found in AntigenDB
No. Hit Found in IEDB

[edit] B cell Epitopes

[edit] BCEpred Analysis

Link to Bcepred
Result Predicited by [2]

[edit] ABCpred Analysis

Link to ABCpred
Result Predicited by [3]

[edit] IEDB Analysis

Link to IEDB
Result Predicited by [4]

[edit] MHC Class-I Binder

[edit] nHLAPred Analysis

Link to nHLApred
Result Predicited by [5]

[edit] IEDB Analysis

Link to IEDB
Result Predicted by [6]

[edit] MHC Class-II Binder

[edit] Propred Analysis

Link to Propred
Result Predicted by [7]

[edit] NetMHC-II Analysis

Link to NetMHC-II
Result Predicted by [8]

[edit] External Links

• Database of Mycobacterium tuberculosis genome sequences and related information.