From DrugPedia: A Wikipedia for Drug discovery

Rv0350
Name
molecular chaperone DnaK
Identifiers
Swiss Prot	P0A5B9
Genbank	885946
PDB	?
Chemical data
Formula	C18H21NO3 [1]
Mol. wt.	66832 Da
Pharmacokinetic data
Bioavailability	?
Solubility	?
Isoelectric-Point	4.8

Contents 1 General 1.1 Protein Sequence 2 Human Homologue Blast Result 3 Alergen Protein 4 Bacterial Toxin Prediction 5 Subcellular Location 6 Antigens 6.1 B cell Epitopes 6.1.1 BCEpred Analysis 6.1.2 ABCpred Analysis 6.1.3 IEDB Analysis 7 MHC Class-I Binder 7.1 nHLAPred Analysis 7.2 IEDB Analysis 8 MHC Class-II Binder 8.1 Propred Analysis 8.2 NetMHC-II Analysis 9 External Links

[edit] General

ACTS AS A CHAPERONE. INVOLVED IN INDUCTION BY STRESS CONDITIONS e.g. HEAT SHOCK. POSSIBLY HAS AN ATPASE ACTIVITY. SEEMS TO BE REGULATED POSITIVELY BY SIGH (Rv3223c PRODUCT) AND NEGATIVELY BY HSPR (Rv0353 PRODUCT).

Conserved hypothetical proline rich protein

Essential gene by Himar1-based transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003)

[edit] Protein Sequence

>Rv0350, TB.seq 419833:421707 MW:66832 MARAVGIDLGTTNSVVSVLEGGDPVVVANSEGSRTTPSIVAFARNGEVLVGQPAKNQAVTNVDRTVRSVKRHMGSDWSIE IDGKKYTAPEISARILMKLKRDAEAYLGEDITDAVITTPAYFNDAQRQATKDAGQIAGLNVLRIVNEPTAAALAYGLDKG EKEQRILVFDLGGGTFDVSLLEIGEGVVEVRATSGDNHLGGDDWDQRVVDWLVDKFKGTSGIDLTKDKMAMQRLREAAEK AKIELSSSQSTSINLPYITVDADKNPLFLDEQLTRAEFQRITQDLLDRTRKPFQSVIADTGISVSEIDHVVLVGGSTRMP AVTDLVKELTGGKEPNKGVNPDEVVAVGAALQAGVLKGEVKDVLLLDVTPLSLGIETKGGVMTRLIERNTTIPTKRSETF TTADDNQPSVQIQVYQGEREIAAHNKLLGSFELTGIPPAPRGIPQIEVTFDIDANGIVHVTAKDKGTGKENTIRIQEGSG LSKEDIDRMIKDAEAHAEEDRKRREEADVRNQAETLVYQTEKFVKEQREAEGGSKVPEDTLNKVDAAVAEAKAALGGSDI SAIKSAMEKLGQESQALGQAIYEAAQAASQATGAAHPGGEPGGAHPGSADDVVDAEVVDDGREAK

[edit] Human Homologue Blast Result

subject ids	% identity	% positives	alignment length	evalue
sp|P38646.2	54	70	621	0.0
sp|P11021.2	50	65	614	4e-163
sp|P11142.1	51	67	558	6e-153
sp|P17066.2	48	63	612	3e-151
sp|P08107.5	50	67	559	1e-150

[edit] Alergen Protein

Link to Algpred
ALLERGEN (By ARPs BLAST method)

[edit] Bacterial Toxin Prediction

Link to btxpred
No Hit Fountd by btxpred server.

[edit] Subcellular Location

Link to TBpred
Integral Membrane

[edit] Antigens

No Hit Found in AntigenDB
No. Hit Found in IEDB

[edit] B cell Epitopes

[edit] BCEpred Analysis

Link to Bcepred
Result Predicited by [2]

[edit] ABCpred Analysis

Link to ABCpred
Result Predicited by [3]

[edit] IEDB Analysis

Link to IEDB
Result Predicited by [4]

[edit] MHC Class-I Binder

[edit] nHLAPred Analysis

Link to nHLApred
Result Predicited by [5]

[edit] IEDB Analysis

Link to IEDB
Result Predicted by [6]

[edit] MHC Class-II Binder

[edit] Propred Analysis

Link to Propred
Result Predicted by [7]

[edit] NetMHC-II Analysis

Link to NetMHC-II
Result Predicted by [8]

[edit] External Links

• Database of Mycobacterium tuberculosis genome sequences and related information.