PolysacDB Tetrasaccharide 2001
From DrugPedia: A Wikipedia for Drug discovery
[edit] Carbohydrate Name
Tetrasaccharide
[edit] Carbohydrate Class
Capsular polysaccharide
[edit] Source Microbe
Streptococcus pneumoniae serotype 6B
[edit] Basic Structure
The structure of the trisaccharide is α-L-Rhap-(1-->4)-D-Rib-ol-(5-->P-->2)-α-D-Galp-(1-->3)-α-D-Glcp-[1-->O(CH2)3NH2]
[edit] Proposed functions
N/A
[edit] Antigenic Nature used to produce antibodies
Glycoconjugates
[edit] Carrier Name
Keyhole limpet hemocyanin
[edit] Conjugation Method
The saccharides were coupled via the 3-aminopropyl spacer to carrier protein KLH in a stepwise reaction: the amino-terminal groups of the OS fragment and KLH were activated by S acetylation and Br acetylation, respectively, and coupled via a thioether linkage
[edit] Antibodies
Antisera
[edit] Antibody type and class
N/A
[edit] Assay System
ELISA, phagocytosis assays and protection experiments
[edit] Cross-reactivity
This antisera cross-reacted with capsular polysaccharide of 6A strains
[edit] Proposed epitopes
N/A
[edit] Proposed Utility
This antisera could passively protect mice against a 6B and 6A challenge
