PolysacDB Ssp-4 (amastigote surface glycoprotein) 2490
From DrugPedia: A Wikipedia for Drug discovery
[edit] Carbohydrate Name
Ssp-4 (amastigote surface glycoprotein)
[edit] Carbohydrate Class
Miscellaneous
[edit] Source Microbe
Trypanosoma cruzi amastigotes
[edit] Basic Structure
Ssp-4 is an amastigote-specific 84 KDa surface component defined by monoclonal antibody (MAb) 2C2, that was shown to be linked to the membrane by a GPI anchor. The epitope defined by MAb 2C2 is homogeneously distributed over the amastigote surface throughout the intracellular life cycle until it is gradually shed through a phospholipase-dependent mechanism during transformation into trypomastigote
[edit] Proposed functions
N/A
[edit] Antigenic Nature used to produce antibodies
mixtures of heat-inactivated intracellular and extracellular amastigotes
[edit] Carrier Name
nil
[edit] Conjugation Method
nil
[edit] Antibodies
Mab 1D9
[edit] Antibody type and class
IgG3
[edit] Assay System
ELISA, immunoblotting, immuno-electron microscopy and confocal immunofluorescence
[edit] Cross-reactivity
This Mab cross-reacts with membrane of amastigotes from 569, 588, 573, 587 and SC2005 isolates, similar to the G isolate. It also reacts with the CL isolate
[edit] Proposed epitopes
N/A
[edit] Proposed Utility
This Mab reacted with cytoplasmic membrane-bound compartments of the amastigotes. This Mab specifically inhibited cell invasion by G and 588, but not CL strains
