PolysacDB O-polysaccharide 2290
From DrugPedia: A Wikipedia for Drug discovery
[edit] Carbohydrate Name
O-polysaccharide
[edit] Carbohydrate Class
O-polysaccharide [LPS]
[edit] Source Microbe
Shigeila sonnei serotype D
[edit] Basic Structure
Chemically, the O-antigen is a polysaccharide chain of variable length, built up by repetitive sequences of tetra- to hexasaccharides. It consists of a basic tetrasaccharide repeating unit consisting of three rhamnoses and one N-acetylglucosamine was common to all S. flexneri strains (except serotype 17): -2)-α-L-Rhap-(1-->2)-α-L-Rhap-(1-->3)-α-L-Rhap-(1-->3)-β-D-GlcpNAc-(1-->
[edit] Proposed functions
N/A
[edit] Antigenic Nature used to produce antibodies
Heat killed cells
[edit] Carrier Name
nil
[edit] Conjugation Method
nil
[edit] Antibodies
Mab Sh5S
[edit] Antibody type and class
IgM
[edit] Assay System
ELISA and immunoblotting
[edit] Cross-reactivity
O-polysaccharide-specific MAb Sh5S recognized LPS molecules from different phase I strains of S. sonnei as well as from strains M51 and C27 of Plesiomonas shigelloides, reported to belong to the same O serotype as S. sonnei. Furthermore, O-antigen determinants expressed by the S. typhi hybrid strain TY37 were recognized.
[edit] Proposed epitopes
N/A
[edit] Proposed Utility
This Mab has been used to analyze clinical isolates and a hybrid Salmonella typhi strain for their expression of the corresponding LPS moieties. In addition to their use in the screening of candidate vaccine strains, the new MAb provide a powerful tool for epidemiological and phylogenetic studies of natural enterobacterial populations
