PolysacDB O-polysaccharide 1247
From DrugPedia: A Wikipedia for Drug discovery
[edit] Carbohydrate Name
O-polysaccharide
[edit] Carbohydrate Class
O-polysaccharide [LPS]
[edit] Source Microbe
Brucella melitensis 16M
[edit] Basic Structure
O-polysaccharide is a homopolymer of 4,6-dideoxy-4-formamidoct-D-mannopyranose residues
[edit] Proposed functions
N/A
[edit] Antigenic Nature used to produce antibodies
B.melitensis 16M formaldehyde-killed cells
[edit] Carrier Name
nil
[edit] Conjugation Method
nil
[edit] Antibodies
Mab BM3-7
[edit] Antibody type and class
IgG2b
[edit] Assay System
ELISA, immunodiffusion and competitive inhibition assay
[edit] Cross-reactivity
This antibody was specific to B. melitiensis and did not cross-reac with O-polysaccharide of B. abortus and Yersinia enterocolitica O:9 polysaccharide
[edit] Proposed epitopes
This Mab reacted only with M antigen. The crucial element of the M epitope is a disaccharide, α-D-Rha4NFo(1-->3)α-D Rha4NFo, that requires adjacent α1,2-linked residues for full activity. The common epitope of A and M antigens is a linear α1,2-linked tri or tetrasaccharide
[edit] Proposed Utility
This Mab can be used as a use as standard Brucella typing or detection reagents
