PolysacDB N-propionyl substituted Neisseria meningitidis group B capsular polysaccharide 1597
From DrugPedia: A Wikipedia for Drug discovery
[edit] Carbohydrate Name
N-propionyl substituted Neisseria meningitidis group B capsular polysaccharide
[edit] Carbohydrate Class
Capsular polysaccharide
[edit] Source Microbe
Group B Neisseria meningitidis
[edit] Basic Structure
It is a homopolymer of α(2-->8) sialic acid
[edit] Proposed functions
This capsular polysaccharide is an important virulence determinant. Serum antibodies to the group B polysaccharide confers protection against disease by activating complement-mediated bacteriolysis and/or opsonization. It leads to immunologic tolerance induced by prenatal exposure to host polysialyated glycoproteins
[edit] Antigenic Nature used to produce antibodies
Glycoconjugates
[edit] Carrier Name
Tetanus toxoid
[edit] Conjugation Method
The oligosaccharide-protein conjugate was prepared by stirring a mixture of terminal aldehyde-containing N-Pr meningococcal B oligosaccharide with tetanus toxoid in 0.75 M potassium phosphate buffer, pH 9.0, with sodium cyanoborohydride (40 mg/ml) for 1 day at 40C, then 2 days at ambient temperature
[edit] Antibodies
Mab Seam 29
[edit] Antibody type and class
IgG2a
[edit] Assay System
ELISA, flow cytometry and indirect immunofluorescence
[edit] Cross-reactivity
This Mab cross-reacted with non-modified acetylaed group B capsular polysaccharide. This Mab also cross-reacted with polysialic acid on CHP-134 neuroblastoma cells
[edit] Proposed epitopes
N/A
[edit] Proposed Utility
This Mab may be useful for the identification of molecular mimetics capable of eliciting protective antibodies specific to the bacteria, without the risk of evoking autoimmune disease
