PolysacDB N-propionyl substituted Neisseria meningitidis group B capsular polysaccharide 1584
From DrugPedia: A Wikipedia for Drug discovery
[edit] Carbohydrate Name
N-propionyl substituted Neisseria meningitidis group B capsular polysaccharide
[edit] Carbohydrate Class
Capsular polysaccharide
[edit] Source Microbe
Group B Neisseria meningitidis
[edit] Basic Structure
It is a homopolymer of α(2-->8) sialic acid
[edit] Proposed functions
This capsular polysaccharide is an important virulence determinant. Serum antibodies to the group B polysaccharide confers protection against disease by activating complement-mediated bacteriolysis and/or opsonization. It leads to immunologic tolerance induced by prenatal exposure to host polysialyated glycoproteins
[edit] Antigenic Nature used to produce antibodies
Glycoconjugates
[edit] Carrier Name
Tetanus toxoid
[edit] Conjugation Method
The oligosaccharide-protein conjugate was prepared by stirring a mixture of terminal aldehyde-containing N-Pr meningococcal B oligosaccharide with tetanus toxoid in 0.75 M potassium phosphate buffer, pH 9.0, with sodium cyanoborohydride (40 mg/ml) for 1 day at 40C, then 2 days at ambient temperature
[edit] Antibodies
Mab Seam 15
[edit] Antibody type and class
IgG2b
[edit] Assay System
ELISA, flow cytometry and indirect immunofluorescence
[edit] Cross-reactivity
This Mab cross-reacted with non-modified acetylaed group B capsular polysaccharide. This Mab also cross-reacted with polysialic acid on CHP-134 neuroblastoma cells
[edit] Proposed epitopes
N/A
[edit] Proposed Utility
This Mab may be useful for the identification of molecular mimetics capable of eliciting protective antibodies specific to the bacteria, without the risk of evoking autoimmune disease. This Mab induced bactericidal activity with rabbit complement
