PolysacDB Lipopolysaccharide 2506
From DrugPedia: A Wikipedia for Drug discovery
[edit] Carbohydrate Name
Lipopolysaccharide
[edit] Carbohydrate Class
Lipopolysaccharide
[edit] Source Microbe
Vibrio cholerae 0139 serogroup
[edit] Basic Structure
The O-antigen part consists of (1-->2)-α-linked 4-amino-4,6-dideoxy-D-mannose (perosamine) whose amino group is acylated with 3-deoxy-L-glycero-tetronic acid. The terminal sugar in the Inaba O-antigen has a hydroxyl at the 2' position
[edit] Proposed functions
V. cholerae lipopolysaccharide (LPS), a critical component of the outer membrane that is required for virulence, is a known target for immune responses following infection or immunization. Antibodies specific for V. cholerae LPS are correlated with protection against cholera
[edit] Antigenic Nature used to produce antibodies
acetone-treated whole cells
[edit] Carrier Name
nil
[edit] Conjugation Method
nil
[edit] Antibodies
Mab ICL12
[edit] Antibody type and class
IgG2b
[edit] Assay System
enzyme-linked immunosorbent assay, slide agglutination test, motility inhibition test, and indirect immunofluorescence test
[edit] Cross-reactivity
This Mab was specific to V. cholerae O139. No cross-reactions were seen with LPS from V cholerae 01 Inaba and Ogawa serotypes
[edit] Proposed epitopes
N/A
[edit] Proposed Utility
All 86 cases diagnosed as V. cholerae 0139 by a rabbit polyclonal antiserum were also detected by this MAb, establishing its utility as highly sensitive and specific diagnostic reagent
