PolysacDB Lipooligosaccharide 1623
From DrugPedia: A Wikipedia for Drug discovery
[edit] Carbohydrate Name
Lipooligosaccharide
[edit] Carbohydrate Class
Lipopolysaccharide
[edit] Source Microbe
Moraxella catarrhalis Serotype A strain 25238
[edit] Basic Structure
LOS consists of an oligosaccharide and lipid A and is similar to the lipopolysaccharide (LPS) of gramnegative enteric pathogens, but it lacks the O-antigenic side chain of repeating units characteristic of classical LPS. The oligosaccharide part consists of the following residues : α-D-Galp-(1-->4)-β-D-Galp-(1-->4)-α-D-Glcp-(1-->2)-β-D-Glcp-(1-->6)-α-D-Glcp [branched to α-D-GlcpNAc-(1-->2)-β-D-Glcp-(1-->4)] and [branched to β-D-Glcp-(1-->3)] -(1-->5)-Kdo
[edit] Proposed functions
Moracella lipooligosaccharide is a potential virulence factor
[edit] Antigenic Nature used to produce antibodies
Glycoconjugates
[edit] Carrier Name
Cross-reactive mutant (CRM9) of diphtheria toxin
[edit] Conjugation Method
Adipic acid dihydrazide (ADH) was bound to detoxified lipooligosaccharide [LOS] to form adipic hydrazide (AH)-dLOS derivatives, using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide HCl (EDC) and N-hydroxysulfosuccinimide (sulfo-NHS). dLOS (70 mg) was dissolved in 7 ml of 345 mM ADH (molar ratio of ADH to LOS is 100 to 1, based on an estimated Mr of 3,000 for dLOS). Sulfo-NHS was added to a concentration of 8 mM, the pH was adjusted to 4.8, and EDC was added to a concentration of 0.1 M. The reaction mixture was stirred and maintained at pH 4.8 for 3 h. The reaction mixture was adjusted to pH 7.0 and passed through the G-50 column as described above. The eluate was assayed for carbohydrate and for AH. The peaks containing both carbohydrate and AH were pooled, freeze-dried, and designated AH-dLOS. AH-dLOS was measured for its composition, using dLOS and ADH as standards
[edit] Antibodies
Polysera
[edit] Antibody type and class
N/A
[edit] Assay System
enzyme-linked immunospot assay, ELISA and Bacterial challenge assay
[edit] Cross-reactivity
N/A
[edit] Proposed epitopes
N/A
[edit] Proposed Utility
The dLOS-CRM vaccine induced a significant bacterial clearance (70 to 90%) of both homologous and heterologous strains in the lungs compared to that observed in the controls
