PolysacDB 2383
From DrugPedia: A Wikipedia for Drug discovery
[edit] Carbohydrate Name
Surface Polysaccharide Poly-N-Acetylglucosamine [PNAG]
[edit] Carbohydrate Class
Miscellaneous
[edit] Source Microbe
Staphylococcus aureus
[edit] Basic Structure
PNAG consists of a backbone of N-acetylglucosamine residues of variable lendth
[edit] Proposed functions
PNAG also referred to as the polysaccharide intercellular adhesin, has been shown to elicit opsonic antibodies when used as a vaccine in goats and rabbits. In addition, these polyclonal antibodies passively protect mice against S. aureus bacteremia and renal infection as well as against lethality following a high-dose infection. Animal antibodies to PNAG also mediate killing of S. epidermidis strains that express this antigen, and these strains constitute a significant proportion of clinical isolates
[edit] Antigenic Nature used to produce antibodies
Staphylococcus aureus infected patients
[edit] Carrier Name
nil
[edit] Conjugation Method
nil
[edit] Antibodies
Mab F598
[edit] Antibody type and class
IgG1
[edit] Assay System
enzyme-linked immunosorbent assays, complement deposition, immunofluorescence and opsonophagocytic assays
[edit] Cross-reactivity
This Mab was specfic to S. aureus and was found to be nonreactive against alginate from Pseudomonas aeruginosa strain PAO 581, the P. aeruginosa LPS O antigens from serogroups 02 and 06, and the PS/A antigen of Bacteroides fragilis
[edit] Proposed epitopes
This Mab bound best to nonacetylated or backbone epitopes on PNAG
[edit] Proposed Utility
This Mab had superior complement deposition and opsonophagocytic activity. This Mab also had optimal protective efficacy. This Mab could be immunotherapeutic agent for preventing or treating staphylococcal infections.
