CarboDB Tetrasaccharide of 3-deoxy-andalpha;-d-manno-oct-2-ulopyranosonic acid (Kdo) 1354
From DrugPedia: A Wikipedia for Drug discovery
[edit] Carbohydrate Name
Tetrasaccharide of 3-deoxy-α-d-manno-oct-2-ulopyranosonic acid (Kdo)
[edit] Carbohydrate Class
Lipopolysaccharide
[edit] Source Microbe
Chlamydophila psittaci
[edit] Basic Structure
Consists of repeating units of tetrasaccharide of 3-deoxy--D-manno-oct-2 ulopyranosonic acid (Kdo) residues
[edit] Proposed functions
LPS represents one of the major surface antigens of this microorganism
[edit] Antigenic Nature used to produce antibodies
Glycoconjugates
[edit] Carrier Name
BSA
[edit] Conjugation Method
The allyl glycosides R-OCH2-CH=CH2 were conjugated with cysteamine yielding R-O(CH2)3-S-(CH2)2-NH3+, which were activated with thiophosgene into the isothiocyanate derivatives R O(CH2)3-S-(CH2)2-N=C=S and then conjugated to BSA yielding R-O(CH2)3-S-(CH2)2-NH-CS-NH-BSA, wherein R represents the glycosyl residue
[edit] Antibodies
Mab S69-4
[edit] Antibody type and class
IgG1
[edit] Assay System
IFT assay, ELISA
[edit] Cross-reactivity
This antibody was specific only to C. psittaci
[edit] Proposed epitopes
The epitope consists of a branched Kdo(2N/A8)[Kdo(2N/A4)] Kdo(2N/A4)Kdo(2N/A4) tetrasaccharide that is specific to Chlamydia psittaci
[edit] Proposed Utility
This Mab may provide insight into the molecular details of the interaction of these antibodies with acidic Kdo containing epitopes. Mab S69-4 can be used in immunohistology to reliably identify C. psittaci at the species level
