CarboDB Smooth Lipopolysaccharide 1220
From DrugPedia: A Wikipedia for Drug discovery
[edit] Carbohydrate Name
Smooth Lipopolysaccharide
[edit] Carbohydrate Class
Lipopolysaccharide
[edit] Source Microbe
Rough Brucella melitensis strain B115
[edit] Basic Structure
The S-LPS of smooth-type Brucella spp. contains two distinct antigenic determinants designated A and M
[edit] Proposed functions
LPS is most unusual constituents of outer membrane. It plays important role in host defence avoidance. LPS contributes to the negative charge of the bacterial surface. Lipid A is often toxic and act as endotoxin
[edit] Antigenic Nature used to produce antibodies
Whole cells
[edit] Carrier Name
nil
[edit] Conjugation Method
nil
[edit] Antibodies
Mab 08C06
[edit] Antibody type and class
IgG3
[edit] Assay System
ELISA and immunoblotting
[edit] Cross-reactivity
This antibody was specific to a common epitope on A and M polysaccharides. But was more reactive towards M dominant strain B. melitensis 16M.This Mab cross-reacted with O-PS of B. abortus 99 but did not bind to R-LPS of B. melitensis B11; also did not cross-react with Y. enterocolitica0:9 LPS
[edit] Proposed epitopes
Both α1-->2 and α1-->3 linkages seem to be an important part of the epitope
[edit] Proposed Utility
this mAb may be particularly useful for typingsmooth Brucella strains
