CarboDB Phosphorylcholine (PC) 2412
From DrugPedia: A Wikipedia for Drug discovery
[edit] Carbohydrate Name
Phosphorylcholine (PC)
[edit] Carbohydrate Class
Miscellaneous
[edit] Source Microbe
Streptococcus pneumoniae
[edit] Basic Structure
N/A
[edit] Proposed functions
Antibodies directed against phosphorylcholine (PC), a major epitope on the cell wall polysaccharide, protect against pneumococcal infections in mice; the role of anti-PC antibodies in humans, however, remains controversial
[edit] Antigenic Nature used to produce antibodies
ASRNKANDYTTEYSASVKGRFIVS [peptide1]
[edit] Carrier Name
BSA
[edit] Conjugation Method
BSA (5 mg) was dissolved in a 0.1 M sodium citrate solution (pH 5.5; 500 ml) to provide a BSA:peptide ratio of 1:25. Glutaraldehyde (0.1%) was added, and the solution was incubated for 1 h at room temperature. The reaction mixture was dialyzed against phosphate-buffered saline (PBS) for 5 days at 4C
[edit] Antibodies
Antisera
[edit] Antibody type and class
N/A
[edit] Assay System
ELISA, Opsonization and protection assays
[edit] Cross-reactivity
N/A
[edit] Proposed epitopes
N/A
[edit] Proposed Utility
This peptide was able to inhibit the binding of anti-PC monoclonal antibodies to PC-BSA
