CarboDB O-polysaccharide 2499
From DrugPedia: A Wikipedia for Drug discovery
[edit] Carbohydrate Name
O-polysaccharide
[edit] Carbohydrate Class
O-polysaccharide [LPS]
[edit] Source Microbe
Vibrio cholerae O1 LPS serotype ogawa 395
[edit] Basic Structure
The O-antigen part consists of (1-->2)-α-linked 4-amino-4,6-dideoxy-D-mannose (perosamine) whose amino group is acylated with 3-deoxy-L-glycero-tetronic acid. The terminal sugar in the Inaba O-antigen has a hydroxyl at the 2' position
[edit] Proposed functions
V. cholerae lipopolysaccharide (LPS), a critical component of the outer membrane that is required for virulence, is a known target for immune responses following infection or immunization. Antibodies specific for V. cholerae LPS are correlated with protection against cholera
[edit] Antigenic Nature used to produce antibodies
Whole cells
[edit] Carrier Name
nil
[edit] Conjugation Method
nil
[edit] Antibodies
Mab 2D6
[edit] Antibody type and class
IgA
[edit] Assay System
ELISA, agglutination assay, indirect immunofluorescence, EM immunogold labeling, invivo protection and immunoblotting
[edit] Cross-reactivity
This Mab is specific to V. cholerae Ogawa and did not cross-react with V. cholerae CA401, a virulent strain of the Inaba serotype
[edit] Proposed epitopes
The nonreducing terminal residue of Ogawa O-PS is the dominant determinant. The C-2 O-methyl group fits into a somewhat flexible antibody cavity and that hydrogen bonds involving the oxygen and, respectively, the OH at the 2- and 3-position of the sugar moiety as well as the 2'-position in the amide side chain are required. [9446585]
[edit] Proposed Utility
Neonatal mice bearing anti-lipopolysaccharide hybridoma backpack tumors were specifically protected against oral challenge
