CarboDB O-polysaccharide 2308
From DrugPedia: A Wikipedia for Drug discovery
[edit] Carbohydrate Name
O-polysaccharide
[edit] Carbohydrate Class
O-polysaccharide [LPS]
[edit] Source Microbe
Shigella flexneri serotype 2a strain 2457T
[edit] Basic Structure
Chemically, the O-antigen is a polysaccharide chain of variable length, built up by repetitive sequences of tetra- to hexasaccharides. It consists of a basic tetrasaccharide repeating unit consisting of three rhamnoses and one N-acetylglucosamine was common to all S. flexneri strains (except serotype 17): -2)-α-L-Rhap-(1-->2)-α-L-Rhap-(1-->3)-α-L-Rhap-(1-->3)-β-D-GlcpNAc-(1-->
[edit] Proposed functions
LPS plays an important virulence role based on its antiphagocytic and endotoxic properties
[edit] Antigenic Nature used to produce antibodies
Whole cells
[edit] Carrier Name
nil
[edit] Conjugation Method
nil
[edit] Antibodies
Mab HYSF2a1
[edit] Antibody type and class
IgG2b
[edit] Assay System
ELISA and immunoblotting
[edit] Cross-reactivity
HySF2a1 reacted only with 2a and 5a LPSs
[edit] Proposed epitopes
This Mab is likely recognizing an epitope influenced by the presence of a single α-D-glycosyl residue on one of the rhamnose residues
[edit] Proposed Utility
This Mab may have implications for designing a Shigella vaccine that will be protective against related serotypes. This Mab may also be used to study the ultrastructural surface distribution of LPSs on the surface of the bacteria
