CarboDB O-polysaccharide 2277
From DrugPedia: A Wikipedia for Drug discovery
[edit] Carbohydrate Name
O-polysaccharide
[edit] Carbohydrate Class
O-polysaccharide [LPS]
[edit] Source Microbe
Shigella flexneri serotype 4a
[edit] Basic Structure
Chemically, the O-antigen is a polysaccharide chain of variable length, built up by repetitive sequences of tetra- to hexasaccharides. It consists of a basic tetrasaccharide repeating unit consisting of three rhamnoses and one N-acetylglucosamine was common to all S. flexneri strains (except serotype 17): -2)-α-L-Rhap-(1-->2)-α-L-Rhap-(1-->3)-α-L-Rhap-(1-->3)-β-D-GlcpNAc-(1-->
[edit] Proposed functions
LPS plays an important virulence role based on its antiphagocytic and endotoxic properties
[edit] Antigenic Nature used to produce antibodies
Whole cells
[edit] Carrier Name
nil
[edit] Conjugation Method
nil
[edit] Antibodies
Mab MASF IV-1
[edit] Antibody type and class
IgG3
[edit] Assay System
ELISA
[edit] Cross-reactivity
MASF IV-1 antibodies recognized all but one of the S. flexneri 4a strains (O antigens IV:3,4) and all serotype 4 strains (O antigen IV only; IV:-), but neither of the two 4b (O antigens IV:6) strains available
[edit] Proposed epitopes
α-D-Glcp-1-->6-β-D-GlcpN residues seemed to be the part of the epitope
[edit] Proposed Utility
This Mab may prove to be valuable reagents in the studies of protective immunity against Shigella bacteria
