CarboDB O-polysaccharide 2060
From DrugPedia: A Wikipedia for Drug discovery
[edit] Carbohydrate Name
O-polysaccharide
[edit] Carbohydrate Class
O-polysaccharide [LPS]
[edit] Source Microbe
Pseudomonas syringae IMV 120a pv. Phaseolicola
[edit] Basic Structure
O-polysaccharides of the LPS consists of tetra- and tri-α-D-rhamnose repeats in the backbone [-3)D-Rha(α1->3)D-Rha(α1-->2)D-Rha(α1->2)D-Rha(α1] and [3)D-Rha(α1-->3)D-Rha(α1-->2)D-Rha(α1-]
[edit] Proposed functions
N/A
[edit] Antigenic Nature used to produce antibodies
Whole cells
[edit] Carrier Name
nil
[edit] Conjugation Method
nil
[edit] Antibodies
Mab Ps1d
[edit] Antibody type and class
IgM
[edit] Assay System
Agglutination, Ouchterlony immunodiffusion, ELISA, SDS-PAGE, and Western immunoblotting
[edit] Cross-reactivity
"This Mab reacted with P. syringae strains with pathovars atrofaciens, phaseolicola and 17 (80%) P. syringae strains for which the pathovar(s) has not been identified"
[edit] Proposed epitopes
The epitope was confined to lateral immunodominant substituents of (α1-->3)D-Rha, (β1-->4)D-GlcNAc, and (α1-->4)D-Fuc, respectively, which are all bound to the backbone-located D-rhamnose
[edit] Proposed Utility
This Mab strongly agglutinated homologous bacterial cells and precipitated soluble antigens
