CarboDB O-polysaccharide 2055

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Contents

[edit] Carbohydrate Name

O-polysaccharide

[edit] Carbohydrate Class

O-polysaccharide [LPS]

[edit] Source Microbe

Pseudomonas syringae IMV 7836 pv. Atrofaciens

[edit] Basic Structure

O-polysaccharides of the LPS consists of tetra- and tri-α-D-rhamnose repeats in the backbone [-3)D-Rha(α1->3)D-Rha(α1-->2)D-Rha(α1->2)D-Rha(α1] and [3)D-Rha(α1-->3)D-Rha(α1-->2)D-Rha(α1-]


[edit] Proposed functions

N/A

[edit] Antigenic Nature used to produce antibodies

Whole cells

[edit] Carrier Name

nil

[edit] Conjugation Method

nil

[edit] Antibodies

Mab Ps1a

[edit] Antibody type and class

IgG3

[edit] Assay System

Agglutination, Ouchterlony immunodiffusion, ELISA, SDS-PAGE, and Western immunoblotting

[edit] Cross-reactivity

"All P. syringae strains with pathovars aptata, glycinea, japonica, phaseolicola, and pisi as well as 72 (87.8%), 3 (75%), and 31 (50%) strains with pathovars atrofaciens, striafaciens, and syringae, respectively, and 20 (80%) P. syringae strains for which the pathovar(s) has not been identified cross-reacted strongly with the MAb Ps1a"

[edit] Proposed epitopes

The epitope of Mab 1a is related to the Rha(α1-->2)D-Rha(α1-->3)D-Rha sequence of the O-polysaccharide backbone with the immunodominant site (α1-->2)D-rhamnose(α1-->3)

[edit] Proposed Utility

This Mab was useful for elucidation of the LPs structure

[edit] Weblink

PubMed Central

[edit] External Links