CarboDB Lipopolysaccharide 2209
From DrugPedia: A Wikipedia for Drug discovery
[edit] Carbohydrate Name
Lipopolysaccharide
[edit] Carbohydrate Class
Lipopolysaccharide
[edit] Source Microbe
Salmonella enteritidis
[edit] Basic Structure
LPS is a complex macromolecule composed of the biologically active lipid A moiety, covalently attached core oligosaccharide, and distally linked polysaccharide O-side chain. The O-antigen part consists of the following residues : -2)-α-D-Manp [branched to α-Tyvp-(1-->3)] -(1-->4)-α-L-Rhap-(1-->3)-α-D-Galp-(1-
[edit] Proposed functions
LPS plays an important virulence role based on its antiphagocytic and endotoxic properties
[edit] Antigenic Nature used to produce antibodies
live attenuated mutant strain of Salmonella typhimurium
[edit] Carrier Name
nil
[edit] Conjugation Method
nil
[edit] Antibodies
Mab 8aC10
[edit] Antibody type and class
N/A
[edit] Assay System
ELISA, immunoblotting, passive hemagglutination (PHA), passive hemolysis and agglutination tests
[edit] Cross-reactivity
The MAb reacted only with lipopolysaccharides (LPS) of Salmonella strains from group O:4 (B), expressing O:5 antigen. The MAb agglutinated in addition Salmonella strains with O:8 antigen from group C(2)-C(3) but did not react with purified LPS
[edit] Proposed epitopes
This Mab is directed to Salmonella O:5 antigen of the LPS. The epitope is possibly localized onto O-acetyl-abequose and abequose residues bound with a α-1-->3 linkage to the basic polysaccharide backbone of Salmonella LPS with O:5 and O:8 antigen respectively
[edit] Proposed Utility
This Mab can be used for identification of Salmonella strains having O:5 LPS
