CarboDB Lipooligosaccharide 1622
From DrugPedia: A Wikipedia for Drug discovery
[edit] Carbohydrate Name
Lipooligosaccharide
[edit] Carbohydrate Class
Lipopolysaccharide
[edit] Source Microbe
Moraxella catarrhalis serotype C strain 26404
[edit] Basic Structure
LOS consists of an oligosaccharide and lipid A and is similar to the lipopolysaccharide (LPS) of gramnegative enteric pathogens, but it lacks the O-antigenic side chain of repeating units characteristic of classical LPS. The oligosaccharide part consists of the following residues : α-D-Galp-(1-->4)-β-D-Galp-(1-->4)-α-D-Glcp-(1-->2)-β-D-Glcp-(1-->6)-α-D-Glcp [branched to β-D-Galp-(1-->4)-α-D-GlcpNAc-(1-->2)-β-D-Glcp-(1-->4)] and [branched to β-D-Glcp-(1-->3)] -(1-->5)-α-Kdop [branched to Kdop-(2-->4)]
[edit] Proposed functions
Moracella lipooligosaccharide is a potential virulence factor
[edit] Antigenic Nature used to produce antibodies
Glycoconjugates
[edit] Carrier Name
Tetanus toxoid
[edit] Conjugation Method
240 mg of lipooligosaccharide [LOS] was detoxified by using anhydrous hydrazine, and the dLOS was purified. Then adipic acid dihydrazide was conjugated to the dLOS (96 mg) in 12 ml of a 287 mM adipic acid dihydrazide suspension to form adipic hydrazide (AH)-dLOS derivatives, using 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide HCl and N-hydroxysulfosuccinimide. The resulting AH-dLOS was finally coupled to Tetanus toxoid [TT]. 10 mg of TT (Mr, 150,000) was reacted with 20 mg of AH-dLOS (10 mg/ml) at a molar ratio of AH-dLOS to TT of 100:1 using 0.05 M 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide HCl. All reaction mixtures were maintained at pH 5.0 to 5.2 for 4 h at 4C, and the reactions were stopped by adjusting the pH to 7.0. The reaction mixtures were dialyzed against 0.9% NaCl for 2 to 3 days, centrifuged, and passed through a Sephacryl S-300 column (2.6 by 90 cm) in 0.9% NaCl. Peaks that contained both protein and carbohydrate were pooled and designated dLOS-TT, dLOS-CRM-1, and dLOS-CRM-2. The three conjugates were analyzed to determine their carbohydrate and protein contents using dLOS and bovine serum albumin as standards
[edit] Antibodies
Polysera
[edit] Antibody type and class
N/A
[edit] Assay System
ELISA, Bactericidal assay and bactericidal inhibition assay
[edit] Cross-reactivity
This antisera cross-reacted with Serotype A LPS and to a little extent to Serotype B
[edit] Proposed epitopes
N/A
[edit] Proposed Utility
This antisera showed complement-mediated bactericidal activity against the homologous strain
