PolysacDB O-polysaccharide 2321
From DrugPedia: A Wikipedia for Drug discovery
[edit] Carbohydrate Name
O-polysaccharide
[edit] Carbohydrate Class
O-polysaccharide [LPS]
[edit] Source Microbe
Shigella flexneri serotype 1b
[edit] Basic Structure
Chemically, the O-antigen is a polysaccharide chain of variable length, built up by repetitive sequences of tetra- to hexasaccharides. It consists of a basic tetrasaccharide repeating unit consisting of three rhamnoses and one N-acetylglucosamine was common to all S. flexneri strains (except serotype 17): -2)-α-L-Rhap-(1-->2)-α-L-Rhap-(1-->3)-α-L-Rhap-(1-->3)-β-D-GlcpNAc-(1-->
[edit] Proposed functions
LPS plays an important virulence role based on its antiphagocytic and endotoxic properties
[edit] Antigenic Nature used to produce antibodies
Heat killed cells
[edit] Carrier Name
nil
[edit] Conjugation Method
nil
[edit] Antibodies
Mab MASF 6-1
[edit] Antibody type and class
IgG1
[edit] Assay System
immunoblotting, enzyme-linked immunosorbent assay (ELISA), hemagglutination, and coagglutination
[edit] Cross-reactivity
This Mab bound to LPSs of S. flexneri serotypes lb, 3a, 3b, and 4b
[edit] Proposed epitopes
These LPSs have one antigenic determinant i.e group 6 antigen. It is therefore likely that the structure responsible for binding is the O-acetyl group on C-2 of rhamnose III
[edit] Proposed Utility
This Mab can be used for detection and identification of shigella flexneri species
