PolysacDB Lipopolysaccharide 2526
From DrugPedia: A Wikipedia for Drug discovery
[edit] Carbohydrate Name
Lipopolysaccharide
[edit] Carbohydrate Class
Lipopolysaccharide
[edit] Source Microbe
Vibrio cholerae 01 serogroup Inaba strain X19203
[edit] Basic Structure
The O-antigen part consists of (1-->2)-α-linked 4-amino-4,6-dideoxy-D-mannose (perosamine) whose amino group is acylated with 3-deoxy-L-glycero-tetronic acid. The terminal sugar in the Inaba O-antigen has a hydroxyl at the 2' position
[edit] Proposed functions
V. cholerae lipopolysaccharide (LPS), a critical component of the outer membrane that is required for virulence, is a known target for immune responses following infection or immunization. Antibodies specific for V. cholerae LPS are correlated with protection against cholera
[edit] Antigenic Nature used to produce antibodies
Killed cells
[edit] Carrier Name
nil
[edit] Conjugation Method
nil
[edit] Antibodies
Mab IH 13
[edit] Antibody type and class
IgG3
[edit] Assay System
slide agglutination, microdilution agglutination, and passive hemagglutination tests
[edit] Cross-reactivity
This Mab reacted only to Inaba-type cells; no agglutination was observed for Ogawa-type cells in either the slide agglutination or the microdilution agglutination tests. This Mab did not show cross-reactivity to Brucella abortus and Yersinia enterocolitica 09
[edit] Proposed epitopes
N/A
[edit] Proposed Utility
This Mab showed strong agglutination with both live cells and heat-killed cells. This Mab will be a very useful tool for resolution of the antigenic structure of V. cholerae 01, especially the Inaba-specific determinant, as well as crossreactive structure to B. abortus. Moreover, highly Inabaspecific antibodies will be able to be used in the identification of Inaba serotypes instead of polyclonal Inaba-specific antisera
