PolysacDB De-O-acetylated Glucurunoxylomannan 2638
From DrugPedia: A Wikipedia for Drug discovery
[edit] Carbohydrate Name
De-O-acetylated Glucurunoxylomannan
[edit] Carbohydrate Class
Miscellaneous
[edit] Source Microbe
Cryptococcus neoformans serotype A
[edit] Basic Structure
A linear α(1-->3)- linked mannan backbone singly substituted with non-reducing β(1-->2) xylose and β(1-->2) glucuronic acid side branches ; O-acetyl groups are present at C-6 of the mannosyl residues
[edit] Proposed functions
Antiphagocytic ; an important virulence factor
[edit] Antigenic Nature used to produce antibodies
Glycoconjugates
[edit] Carrier Name
Sheep erythrocytes
[edit] Conjugation Method
The capsular polysaccharide was adsorbed with 0.1 ml of packed sheep erythrocytes for 2 hours at room temperature
[edit] Antibodies
Mab F12D2
[edit] Antibody type and class
IgG3
[edit] Assay System
ELISA
[edit] Cross-reactivity
This antibody cross-reacted with Serotypes A, B, C and D
[edit] Proposed epitopes
Studies on this antibody showed that there are 2 types of epitopes on GXM-O-acetyl dependent and O-acetyl independent. MAb F12D2 also fails to react with xylose deficient GXM shopwing that xylose residues form an important part of the epitope
[edit] Proposed Utility
This antibody was used in the elucidation of the probable epitopes present in glucurunoxylomannan. This antibody showed that the induction of a MAb that is reactive with all serotypes does not inherently require the presence of an O-acetyl group on the immunizing GXM
