PolysacDB De-O-acetylated Glucurunoxylomannan 2638
From DrugPedia: A Wikipedia for Drug discovery
Carbohydrate Name
De-O-acetylated Glucurunoxylomannan
Carbohydrate Class
Miscellaneous
Source Microbe
Cryptococcus neoformans serotype A
Basic Structure
A linear α(1-->3)- linked mannan backbone singly substituted with non-reducing β(1-->2) xylose and β(1-->2) glucuronic acid side branches ; O-acetyl groups are present at C-6 of the mannosyl residues
Proposed functions
Antiphagocytic ; an important virulence factor
Antigenic Nature used to produce antibodies
Glycoconjugates
Carrier Name
Sheep erythrocytes
Conjugation Method
The capsular polysaccharide was adsorbed with 0.1 ml of packed sheep erythrocytes for 2 hours at room temperature
Antibodies
Mab F12D2
Antibody type and class
IgG3
Assay System
ELISA
Cross-reactivity
This antibody cross-reacted with Serotypes A, B, C and D
Proposed epitopes
Studies on this antibody showed that there are 2 types of epitopes on GXM-O-acetyl dependent and O-acetyl independent. MAb F12D2 also fails to react with xylose deficient GXM shopwing that xylose residues form an important part of the epitope
Proposed Utility
This antibody was used in the elucidation of the probable epitopes present in glucurunoxylomannan. This antibody showed that the induction of a MAb that is reactive with all serotypes does not inherently require the presence of an O-acetyl group on the immunizing GXM
