PolysacDB Capsular polysaccharide 2605
From DrugPedia: A Wikipedia for Drug discovery
[edit] Carbohydrate Name
Capsular polysaccharide
[edit] Carbohydrate Class
Capsular polysaccharide
[edit] Source Microbe
Cryptococcus neoformans Serotype D
[edit] Basic Structure
The capsular polysaccharide has a linear α-(1-->3)-linked mannose backbone that is substituted with nonreducing D-xylosyl and D-glucuronosyl acid groups. The mannose backbone is partially O-acetylated
[edit] Proposed functions
The capsular polysaccharide is an important virulence factor
[edit] Antigenic Nature used to produce antibodies
Glycoconjugates
[edit] Carrier Name
Keyhole limpet hemocyanin
[edit] Conjugation Method
Forty milligrams of crude polysaccharide was dissolved in 4 ml of physiological saline solution (PSS). One milliliter of 1% cyanuric chloride in dimethylformamide was added to the polysaccharide solution and stirred for 1 h at 48C. KLH in PSS (100 mg/2ml) was added and stirred for 1 h at 258C, and the solution was then incubated at 48C for 18 h. The solution was dialyzed against water for 30 h and centrifuged. The protein concentration in the supernatant was adjusted to 2 mg/ml, 1/9 volume of 10% aluminum potassium sulfate solution was added, and the pH was adjusted to 6.5 with 10% sodium carbonate. The precipitated antigen was removed by centrifugation and resuspended in PSS
[edit] Antibodies
Mab CRND8
[edit] Antibody type and class
IgM
[edit] Assay System
ELISA and CSA[Cell Slide Agglutination]
[edit] Cross-reactivity
This antibody cross-reacted with Serotype A and D serotypes
[edit] Proposed epitopes
O-acetylated glucurunoxylomannan
[edit] Proposed Utility
used to differentiate various strains of all serotypes
