PolysacDB Capsular polysaccharide 2602

From DrugPedia: A Wikipedia for Drug discovery

Contents 1 Carbohydrate Name 2 Carbohydrate Class 3 Source Microbe 4 Basic Structure 5 Proposed functions 6 Antigenic Nature used to produce antibodies 7 Carrier Name 8 Conjugation Method 9 Antibodies 10 Antibody type and class 11 Assay System 12 Cross-reactivity 13 Proposed epitopes 14 Proposed Utility 15 Weblink 16 External Links

[edit] Carbohydrate Name

Capsular polysaccharide

[edit] Carbohydrate Class

Capsular polysaccharide

[edit] Source Microbe

Cryptococcus neoformans Serotype D

[edit] Basic Structure

The capsular polysaccharide has a linear α-(1-->3)-linked mannose backbone that is substituted with nonreducing D-xylosyl and D-glucuronosyl acid groups. The mannose backbone is partially O-acetylated

[edit] Proposed functions

The capsular polysaccharide is an important virulence factor

[edit] Antigenic Nature used to produce antibodies

Glycoconjugates

[edit] Carrier Name

Sheep erythrocytes

[edit] Conjugation Method

The capsular polysaccharide was adsorbed with 0.1 ml of packed sheep erythrocytes for 2 hours at room temperature

[edit] Antibodies

Mab 386

[edit] Antibody type and class

N/A

[edit] Assay System

ELISA and Immunodiffusion

[edit] Cross-reactivity

This antibody has a highly variable cross-reactivity. It cross reacts with most strains of Serotype A and D. It cross reacts with only one strain of serotype B and 2 strains of serotype C. does not show a reaction with ELISA

[edit] Proposed epitopes

Since Mab 386 does not show any positive results with ELISA, it is suggested that binding of polysaccharides to plates coated with poly-L-lysine altered the carboxyl residues that may form part of an epitope recognized by the antibody

[edit] Proposed Utility

used to differentiate various strains of all serotypes

[edit] Weblink

PubMed

[edit] External Links

• BCSDB Structural Detail

• Link to PolysacDB database