PolysacDB Lipopolysaccharide 2551

From DrugPedia: A Wikipedia for Drug discovery

Contents 1 Carbohydrate Name 2 Carbohydrate Class 3 Source Microbe 4 Basic Structure 5 Proposed functions 6 Antigenic Nature used to produce antibodies 7 Carrier Name 8 Conjugation Method 9 Antibodies 10 Antibody type and class 11 Assay System 12 Cross-reactivity 13 Proposed epitopes 14 Proposed Utility 15 Weblink 16 External Links

Carbohydrate Name

Lipopolysaccharide

Carbohydrate Class

Lipopolysaccharide

Source Microbe

Vibrio cholerae 01 35 Inaba

Basic Structure

The O-antigen part consists of (1-->2)-α-linked 4-amino-4,6-dideoxy-D-mannose (perosamine) whose amino group is acylated with 3-deoxy-L-glycero-tetronic acid. The terminal sugar in the Inaba O-antigen has a hydroxyl at the 2' position

Proposed functions

V. cholerae lipopolysaccharide (LPS), a critical component of the outer membrane that is required for virulence, is a known target for immune responses following infection or immunization. Antibodies specific for V. cholerae LPS are correlated with protection against cholera

Antigenic Nature used to produce antibodies

Purified LPS

Carrier Name

nil

Conjugation Method

nil

Antibodies

Mab H4

Antibody type and class

IgM

Assay System

enzyme-linked immunosorbent assay inhibition experiments

Cross-reactivity

This Mab reacted with Ogawa 34 and Inaba 35 serotype LPS but did not react with O2 LPS

Proposed epitopes

This Mab was specific to A antigen. Epitope A was postulated to be either the perosamine residues or the N-tetronic acid (N-3-deoxy-L-glycero-tetronic acid) side chain

Proposed Utility

This Mab had a good agglutinating capacity when tested against V. cholerae 0:1 strains

Weblink

PubMed Central

External Links

• Link to PolysacDB database