PolysacDB Lipopolysaccharide 2508
From DrugPedia: A Wikipedia for Drug discovery
Carbohydrate Name
Lipopolysaccharide
Carbohydrate Class
Lipopolysaccharide
Source Microbe
Vibrio cholerae 01 serogroup
Basic Structure
The O-antigen part consists of (1-->2)-α-linked 4-amino-4,6-dideoxy-D-mannose (perosamine) whose amino group is acylated with 3-deoxy-L-glycero-tetronic acid. The terminal sugar in the Inaba O-antigen has a hydroxyl at the 2' position
Proposed functions
V. cholerae lipopolysaccharide (LPS), a critical component of the outer membrane that is required for virulence, is a known target for immune responses following infection or immunization. Antibodies specific for V. cholerae LPS are correlated with protection against cholera
Antigenic Nature used to produce antibodies
Killed Vaccine consisting of V. cholerae O1 Inaba (ATCC 14033) and Ogawa (ATCC 14035) cells
Carrier Name
nil
Conjugation Method
nil
Antibodies
Mab A6
Antibody type and class
IgG3
Assay System
enzyme-linked immunosorbent assay (ELISA), immunofluorescence, Immunoelectron microscopy and the slide agglutination test
Cross-reactivity
This Mab reacted with both Inaba and Ogawa serovars (A antigen) and was specific to O1 serotype
Proposed epitopes
The epitope was named as Epitope A. Epitope A was postulated to be either the perosamine residues or the N-tetronic acid (N-3-deoxy-L-glycero-tetronic acid) side chain
Proposed Utility
This Mab produced discernible agglutination within 1 min with each of the 37 01 whole-cell suspensions tested
