PolysacDB 2495
From DrugPedia: A Wikipedia for Drug discovery
[edit] Carbohydrate Name
O-polysaccharide [Terminal hexasaccharides]
[edit] Carbohydrate Class
O-polysaccharide [LPS]
[edit] Source Microbe
Vibrio cholerae O1 LPS serotype ogawa
[edit] Basic Structure
The O-antigen part consists of (1-->2)-α-linked 4-amino-4,6-dideoxy-D-mannose (perosamine) whose amino group is acylated with 3-deoxy-L-glycero-tetronic acid. The terminal sugar is characterized by a 2-O-methyl group
[edit] Proposed functions
V. cholerae lipopolysaccharide (LPS), a critical component of the outer membrane that is required for virulence, is a known target for immune responses following infection or immunization. Antibodies specific for V. cholerae LPS are correlated with protection against cholera
[edit] Antigenic Nature used to produce antibodies
Glycoconjugates
[edit] Carrier Name
BSA
[edit] Conjugation Method
The purified carbohydrates were subjected to aminolysis to afford ethylenediamine derivatives, which were treated with squaric acid diethyl ester. The squaric acid monoesters, thus obtained, were treated with BSA to give neoglycoconjugates
[edit] Antibodies
Antisera
[edit] Antibody type and class
IgG and IgM
[edit] Assay System
ELISA
[edit] Cross-reactivity
N/A
[edit] Proposed epitopes
The epitope was named as Epitope A. Epitope A was postulated to be either the perosamine residues or the N-tetronic acid (N-3-deoxy-L-glycero-tetronic acid) side chain
[edit] Proposed Utility
This antisera showed protection
