PolysacDB Phosphorylcholine (PC) 2412

From DrugPedia: A Wikipedia for Drug discovery

Contents 1 Carbohydrate Name 2 Carbohydrate Class 3 Source Microbe 4 Basic Structure 5 Proposed functions 6 Antigenic Nature used to produce antibodies 7 Carrier Name 8 Conjugation Method 9 Antibodies 10 Antibody type and class 11 Assay System 12 Cross-reactivity 13 Proposed epitopes 14 Proposed Utility 15 Weblink 16 External Links

Carbohydrate Name

Phosphorylcholine (PC)

Carbohydrate Class

Miscellaneous

Source Microbe

Streptococcus pneumoniae

Basic Structure

N/A

Proposed functions

Antibodies directed against phosphorylcholine (PC), a major epitope on the cell wall polysaccharide, protect against pneumococcal infections in mice; the role of anti-PC antibodies in humans, however, remains controversial

Antigenic Nature used to produce antibodies

ASRNKANDYTTEYSASVKGRFIVS [peptide1]

Carrier Name

BSA

Conjugation Method

BSA (5 mg) was dissolved in a 0.1 M sodium citrate solution (pH 5.5; 500 ml) to provide a BSA:peptide ratio of 1:25. Glutaraldehyde (0.1%) was added, and the solution was incubated for 1 h at room temperature. The reaction mixture was dialyzed against phosphate-buffered saline (PBS) for 5 days at 4C

Antibodies

Antisera

Antibody type and class

N/A

Assay System

ELISA, Opsonization and protection assays

Cross-reactivity

N/A

Proposed epitopes

N/A

Proposed Utility

This peptide was able to inhibit the binding of anti-PC monoclonal antibodies to PC-BSA

Weblink

PubMed

External Links

• Link to PolysacDB database