PolysacDB O-polysaccharide 2320
From DrugPedia: A Wikipedia for Drug discovery
Carbohydrate Name
O-polysaccharide
Carbohydrate Class
O-polysaccharide [LPS]
Source Microbe
Shigella flexneri serotype 1b
Basic Structure
Chemically, the O-antigen is a polysaccharide chain of variable length, built up by repetitive sequences of tetra- to hexasaccharides. It consists of a basic tetrasaccharide repeating unit consisting of three rhamnoses and one N-acetylglucosamine was common to all S. flexneri strains (except serotype 17): -2)-α-L-Rhap-(1-->2)-α-L-Rhap-(1-->3)-α-L-Rhap-(1-->3)-β-D-GlcpNAc-(1-->
Proposed functions
LPS plays an important virulence role based on its antiphagocytic and endotoxic properties
Antigenic Nature used to produce antibodies
Heat killed cells
Carrier Name
nil
Conjugation Method
nil
Antibodies
Mab MASF 111:6,7,8
Antibody type and class
N/A
Assay System
immunoblotting, enzyme-linked immunosorbent assay (ELISA), hemagglutination, and coagglutination
Cross-reactivity
The type 111:6,7,8 O-antigen-specific antibodies were specific for S. flexneri serotype 3a
Proposed epitopes
These antibodies seemed to require the simultaneous presence of all three antigens for binding i.e. type III antigen and the group 6 and 7,8 antigens
Proposed Utility
This Mab can be used for detection and identification of shigella flexneri species
