PolysacDB O-polysaccharide 2277
From DrugPedia: A Wikipedia for Drug discovery
Carbohydrate Name
O-polysaccharide
Carbohydrate Class
O-polysaccharide [LPS]
Source Microbe
Shigella flexneri serotype 4a
Basic Structure
Chemically, the O-antigen is a polysaccharide chain of variable length, built up by repetitive sequences of tetra- to hexasaccharides. It consists of a basic tetrasaccharide repeating unit consisting of three rhamnoses and one N-acetylglucosamine was common to all S. flexneri strains (except serotype 17): -2)-α-L-Rhap-(1-->2)-α-L-Rhap-(1-->3)-α-L-Rhap-(1-->3)-β-D-GlcpNAc-(1-->
Proposed functions
LPS plays an important virulence role based on its antiphagocytic and endotoxic properties
Antigenic Nature used to produce antibodies
Whole cells
Carrier Name
nil
Conjugation Method
nil
Antibodies
Mab MASF IV-1
Antibody type and class
IgG3
Assay System
ELISA
Cross-reactivity
MASF IV-1 antibodies recognized all but one of the S. flexneri 4a strains (O antigens IV:3,4) and all serotype 4 strains (O antigen IV only; IV:-), but neither of the two 4b (O antigens IV:6) strains available
Proposed epitopes
α-D-Glcp-1-->6-β-D-GlcpN residues seemed to be the part of the epitope
Proposed Utility
This Mab may prove to be valuable reagents in the studies of protective immunity against Shigella bacteria
