PolysacDB O-polysaccharide 2059

From DrugPedia: A Wikipedia for Drug discovery

Contents 1 Carbohydrate Name 2 Carbohydrate Class 3 Source Microbe 4 Basic Structure 5 Proposed functions 6 Antigenic Nature used to produce antibodies 7 Carrier Name 8 Conjugation Method 9 Antibodies 10 Antibody type and class 11 Assay System 12 Cross-reactivity 13 Proposed epitopes 14 Proposed Utility 15 Weblink 16 External Links

Carbohydrate Name

O-polysaccharide

Carbohydrate Class

O-polysaccharide [LPS]

Source Microbe

Pseudomonas syringae NCPPB 2612 pv. Atrofaciens

Basic Structure

O-polysaccharides of the LPS consists of tetra- and tri-α-D-rhamnose repeats in the backbone [-3)D-Rha(α1->3)D-Rha(α1-->2)D-Rha(α1->2)D-Rha(α1] and [3)D-Rha(α1-->3)D-Rha(α1-->2)D-Rha(α1-]

Proposed functions

N/A

Antigenic Nature used to produce antibodies

Whole cells

Carrier Name

nil

Conjugation Method

nil

Antibodies

Mab Ps1c

Antibody type and class

IgM

Assay System

Agglutination, Ouchterlony immunodiffusion, ELISA, SDS-PAGE, and Western immunoblotting

Cross-reactivity

" P. syringae strains with pathovars aptata, glycinea, japonica, pisi, atrofaciens and 1 P. syringae strain for which the pathovar(s) has not been identified cross-reacted strongly with the MAb Ps1c"

Proposed epitopes

The epitope was confined to lateral immunodominant substituents of (α1-->3)D-Rha, (β1-->4)D-GlcNAc, and (α1-->4)D-Fuc, respectively, which are all bound to the backbone-located D-rhamnose

Proposed Utility

This Mab strongly agglutinated homologous bacterial cells and precipitated soluble antigens

Weblink

PubMed Central

External Links

• Link to PolysacDB database