PolysacDB Trisaccharide 2000
From DrugPedia: A Wikipedia for Drug discovery
Carbohydrate Name
Trisaccharide
Carbohydrate Class
Capsular polysaccharide
Source Microbe
Streptococcus pneumoniae serotype 6B
Basic Structure
The structure of the trisaccharide is -D-Rib-ol-(5-->P-->2)-α-D-Galp-(1-->3)-α-D-Glcp-[1-->O(CH2)3NH2]
Proposed functions
N/A
Antigenic Nature used to produce antibodies
Glycoconjugates
Carrier Name
Keyhole limpet hemocyanin
Conjugation Method
The saccharides were coupled via the 3-aminopropyl spacer to carrier protein KLH in a stepwise reaction: the amino-terminal groups of the OS fragment and KLH were activated by S acetylation and Br acetylation, respectively, and coupled via a thioether linkage
Antibodies
Antisera
Antibody type and class
N/A
Assay System
ELISA, phagocytosis assays and protection experiments
Cross-reactivity
This antisera was specific to capsular polysaccharide of strain 6B and did not cross-react with 6A strains
Proposed epitopes
N/A
Proposed Utility
This antisera could passively protect mice against a 6B challenge
