PolysacDB Lipopolysaccharide 1831

From DrugPedia: A Wikipedia for Drug discovery

Contents 1 Carbohydrate Name 2 Carbohydrate Class 3 Source Microbe 4 Basic Structure 5 Proposed functions 6 Antigenic Nature used to produce antibodies 7 Carrier Name 8 Conjugation Method 9 Antibodies 10 Antibody type and class 11 Assay System 12 Cross-reactivity 13 Proposed epitopes 14 Proposed Utility 15 Weblink 16 External Links

[edit] Carbohydrate Name

Lipopolysaccharide

[edit] Carbohydrate Class

Lipopolysaccharide

[edit] Source Microbe

Neisseria gonorrhoeae strain 4505

[edit] Basic Structure

Gonococcal lipopolysaccharide (LPS) is a complex of at least three major antigenic determinants. The ceramide part consists of the following residues : β-D-GalpNAc-(1-->3)-β-D-Galp-(1-->4)-β-D-Glcp-(1-?)-CER-(?--/Ceramide/. The core oligosaccharide part consists of the following residues : α-D-GlcpNAc-(1-->2)-L-gro [branched to β-D-Galp-(1-->4)-β-D-Glcp-(1-->4)] -α-D-manHepp-(1-->3)-L-gro-α-D-manHepp-(1-->5)-Kdo. The other part consists of the following residues : β-D-GalpNAc-(1-->3)-β-D-Galp-(1-->4)-β-D-GlcpNAc-(1-->3)-β-D-Galp-(1-->4)-β-D-Glcp-(1-->4)-L-gro [branched to α-D-GlcpNAc-(1-->2)-L-gro-α-D-manHepp-(1-->3)] -α-D-manHepp-(1-->5)-Kdo

[edit] Proposed functions

It is an important virulence factor

[edit] Antigenic Nature used to produce antibodies

heat-killed gonococcal strain 4505 cells

[edit] Carrier Name

nil

[edit] Conjugation Method

nil

[edit] Antibodies

Mab 3F11

[edit] Antibody type and class

IgG1

[edit] Assay System

ELISA

[edit] Cross-reactivity

This Mab cross-reacted with meningococcal LPS but failed to react with LPS isolated from Escherichia coli 0:111, the J-5 mutant of E. coli 0:111, or Salmonella minnesota R595

[edit] Proposed epitopes

D-galactosamine, α and β lactose residues seemed to be the part of the epitope

[edit] Proposed Utility

This Mab may be used for epitope elucidation of gonococcal LPS

[edit] Weblink

PubMed Central

[edit] External Links

• Link to PolysacDB database