PolysacDB Lipooligosaccharide 1616
From DrugPedia: A Wikipedia for Drug discovery
[edit] Carbohydrate Name
Lipooligosaccharide
[edit] Carbohydrate Class
Lipopolysaccharide
[edit] Source Microbe
Moraxella catarrhalis
[edit] Basic Structure
LOS consists of an oligosaccharide and lipid A and is similar to the lipopolysaccharide (LPS) of gramnegative enteric pathogens, but it lacks the O-antigenic side chain of repeating units characteristic of classical LPS. The oligosaccharide part consists of the following residues : α-D-Galp-(1-->4)-β-D-Galp-(1-->4)-α-D-Glcp-(1-->2)-β-D-Glcp-(1-->6)-α-D-Glcp [branched to α-D-GlcpNAc-(1-->2)-β-D-Glcp-(1-->4)] and [branched to β-D-Glcp-(1-->3)] -(1-->5)-Kdo[ branched to Kdop-(2-?)]
[edit] Proposed functions
Moracella lipooligosaccharide is a potential virulence factor
[edit] Antigenic Nature used to produce antibodies
Whole cells
[edit] Carrier Name
nil
[edit] Conjugation Method
nil
[edit] Antibodies
Mab 8E7
[edit] Antibody type and class
IgG3
[edit] Assay System
ELISA, immunoblotting, Bacterial adherence assay, Bactericidal assay and immunoelectron microscopy
[edit] Cross-reactivity
MAb 8E7 reacted with M. catarrhalis O35E, serotype A and C LOSs but not serotype B LOS
[edit] Proposed epitopes
The epitope recognized by MAb 8E7 is directed to a common sequence of either α-GlcNAc-(1-->2)-β-Glc-(1--> at the branch substituting position 4 of the trisubstituted Glc residue or a terminal tetrasaccharide α-Gal-(1-->4)-β-Gal-(1-->4)-α-Glc-(1-->2)-β-Glc-[1--> at the branch substituting position 6 of the trisubstituted Glc residue
[edit] Proposed Utility
This Mab had had bactericidal activity. Passive immunization with Mab 8E7 could significantly enhance the clearance of strain O35E from mouse lungs in an aerosol challenge mouse model
