PolysacDB FVP2 polysaccharide 1450

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Contents

[edit] Carbohydrate Name

FVP2 polysaccharide

[edit] Carbohydrate Class

Miscellaneous

[edit] Source Microbe

Flammulina velutipes

[edit] Basic Structure

Consists of an α-(1-->4)-D-glucan, branching with a single α-D-glucan at the C-6 position to every seven residues along the main chain


[edit] Proposed functions

FVP2 can enhance the livability of primary culture of mouse hepatocytes in vitro and decrease the release of the alanine aminotransferase (ALT) as well as apoptosis of hepatocytes after the carbon tetrachloride (CCl4) intoxication. Furthermore, FVP2 has the capability of suppressing the growth of hepatoma 22 cells and sarcoma 180 cells (S180), extending the survival time of L615 leukemia mice, and promoting the lymphocyte transformation rate, natural killer cell activity, and the interleukin-2 production index in normal and tumor-bearing (S180) mice

[edit] Antigenic Nature used to produce antibodies

Glycoconjugates

[edit] Carrier Name

Bovine serum albumin

[edit] Conjugation Method

The FVP2 polysaccharide was conjugated to BSA using 1-cyano-4-dimethyl-aminopyridinium tetrafluoroborate (CDAP) treatment

[edit] Antibodies

Mab B11D

[edit] Antibody type and class

IgM

[edit] Assay System

Indirect ELISA, Double Immunodiffusion

[edit] Cross-reactivity

N/A

[edit] Proposed epitopes

N/A

[edit] Proposed Utility

This Mab provides a potentially useful tool for further study in the development of a novel and efficient immunoassay to detect FVP2 in biological specimens

[edit] Weblink

PubMed

[edit] External Links