PolysacDB Lipoologosaccharide band A of the LPS 1171

From DrugPedia: A Wikipedia for Drug discovery

Contents 1 Carbohydrate Name 2 Carbohydrate Class 3 Source Microbe 4 Basic Structure 5 Proposed functions 6 Antigenic Nature used to produce antibodies 7 Carrier Name 8 Conjugation Method 9 Antibodies 10 Antibody type and class 11 Assay System 12 Cross-reactivity 13 Proposed epitopes 14 Proposed Utility 15 Weblink 16 External Links

Carbohydrate Name

Lipoologosaccharide band A of the LPS

Carbohydrate Class

Lipopolysaccharide

Source Microbe

Bordetella pertusis

Basic Structure

B. pertusis LPS is resolved electrophoretically into two bands, a slowly migrating band designated LOS A and a fast-migrating band designated LOS B. These two species differ in molecular weight as three N-acetyl amino sugars found on LOS A are not present on LOS B

Proposed functions

The LOS of B. pertussis exhibits biological activities similar to those of endotoxins from other gram-negative bacteria, including pyrogenicity, toxicity, induction of the Shwartzman reaction, induction of tumor necrosis factor production, and adjuvant properties

Antigenic Nature used to produce antibodies

N/A

Carrier Name

N/A

Conjugation Method

N/A

Antibodies

Mab G10F8C3

Antibody type and class

IgG3

Assay System

Immunoblotting, Protein A sepharose affinity chromatography, bactericidal assays

Cross-reactivity

This antibody was specific to B. pertusis strains

Proposed epitopes

This Mab reacts with the core oligosaccharide moeity

Proposed Utility

This Mab exhibits complement dependent bactericidal activity against strains of B. pertussis that express the LOS A phenotype. In addition it was effective in reducing colonization by B. pertussis in both the lungs and tracheas of mice after aerosol infection

Weblink

PubMed Central

External Links

• Link to PolysacDB database