PolysacDB O-polysaccharide 1106
From DrugPedia: A Wikipedia for Drug discovery
[edit] Carbohydrate Name
O-polysaccharide
[edit] Carbohydrate Class
O-polysaccharide [LPS]
[edit] Source Microbe
Actinobacillus pleuropneumoniae serotype 4 (field isolate F6)
[edit] Basic Structure
O chains of LPS of serotypes 4 and 7 were structurally similar, being composed of repeating tetrasaccharide units with identical trisaccharide residues forming a common backbone
[edit] Proposed functions
LPS is an important virulence factor. It molecule plays an important role in adherence of the bacterium to porcine respiratory tract cells and mucus
[edit] Antigenic Nature used to produce antibodies
formalin-fixed A. pleuropneumoniae serotype 4 cells
[edit] Carrier Name
nil
[edit] Conjugation Method
nil
[edit] Antibodies
Mab 3E12
[edit] Antibody type and class
IgM
[edit] Assay System
Whole cell ELISA and slide agglutination tests
[edit] Cross-reactivity
This Mab failed to recognize surface epitopes located on other bacterial, such as H. parasuis, P. haemolytica, P. multocida capsular types A and D, Haemophilus taxon minor group, and A. suis, K. pneumoniae and B. bronchiseptica. were reactive with 26 serotype 4 isolates,11 serotype 7 isolates, and 1 each of the serotype 1 and non-typeable isolates tested
[edit] Proposed epitopes
N/A
[edit] Proposed Utility
This MAb could be useful for the serological diagnostic of animals infected with serotypes 4 and 7 by inhibition ELISA or competition ELISA. Moreover, they could be useful as complementary reagents to polyclonal sera for serotyping of field isolates belonging to A. pleuropneumoniae serotype 4 or 7
