PolysacDB Glucan 1014

From DrugPedia: A Wikipedia for Drug discovery

Contents 1 Carbohydrate Name 2 Carbohydrate Class 3 Source Microbe 4 Basic Structure 5 Proposed functions 6 Antigenic Nature used to produce antibodies 7 Carrier Name 8 Conjugation Method 9 Antibodies 10 Antibody type and class 11 Assay System 12 Cross-reactivity 13 Proposed epitopes 14 Proposed Utility 15 Weblink 16 External Links

[edit] Carbohydrate Name

Glucan

[edit] Carbohydrate Class

Miscellaneous

[edit] Source Microbe

Candida albicans

[edit] Basic Structure

Glucans are a heterogeneous group of glucose polymers, consisting of a backbone of β(1-->3)-linked β-D-glucopyranosyl units with β(1-->6)-linked side chains of varying distribution and length

[edit] Proposed functions

Glucan is an essential cell wall component in pathogenic fungi and plays a critical role in cell viability

[edit] Antigenic Nature used to produce antibodies

Glycoconjugates

[edit] Carrier Name

Diphtheria toxoid CRM 197

[edit] Conjugation Method

A 20-fold molar excess of activated polysaccharide was reacted overnight with the protein, at room temperature, in 10 mM phosphate buffer, pH 7.2. The unbound polysaccharide was separated from the glyco-conjugate by ultrafiltration, using Amicon 10-kD filter devices

[edit] Antibodies

Mab 2G8

[edit] Antibody type and class

IgG2

[edit] Assay System

Flow Cytometry, confocal microscopy, phospholipase assay, yeast growth inhibition assay

[edit] Cross-reactivity

This antibody binds both C. neoformans and Candida albicans

[edit] Proposed epitopes

N\A

[edit] Proposed Utility

MAb 2G8 inhibits the in vitro growth of C. neoformans and also exerts anti-C. neoformans protective effects in vivo.

[edit] Weblink

PubMed Central

[edit] External Links

• BCSDB Structural Detail

• IEDB Epitopic Detail

• Link to PolysacDB database